Vector construction, lentiviral production and titering      2-3 weeks

add 2-4 weeks for gene synthesis

Lentiviral production and titering                            5-7 days

Transduction and generate stable cell lines         2-4 wees

Single cell cloning in 96-well plates                        2-4 weeks

Transfer colonies to 6-well plates                           1-2 weeks

Cell expansion and make frozen stocks                2-4 weeks

RNA isolation + RT-PCR                                             5-7 days

WB/FACS, ELISA                                                          3-7 days

Cell-based assay                                                         1-2 weeks

Recombinant protein production                           4-10 weeks