The Ba/F3 cell line is mouse pro-B cell requiring interleukin-3 (mIL3) for survival and proliferation.  Ba/F3 cells may grow without mIL3 while the Ba/F3 cell line transduced and transformed by an oncogene.  Therefore, the Ba/F3 cell line has been a popular system to evaluate oncogenicity of genes and mutations, and efficacies of targeted drugs.

Biosettia have established a workflow including cloning the genes of interest and mutants, generating lentiviruses and stable lines, mIL3 withdrawal to confirm the oncogenicity and drug screening.