Lentiviral Packaging Mix (Catalog# pLV-PACK-500/1000): the packaging mix for the lentiviral vectors with a chimeric 5’ LTR

The optimized packaging vector mix provides HIV-1 gag, pol, and rev gene products and vesicular stomatitis virus G (VSV-G) protein encoded in three plasmids (Fig. 1).  This packaging mix is good for all of Biosettia lentiviral vectors but cannot be used in packaging lentiviral vectors with a wildtype 5′ LTR due to lack of Tat gene in the packaging vectors.

Diagram showing three circular plasmids labeled pLV-PACK-1, pLV-PACK-2, and pLV-PACK-3, with respective sizes 8.9 kb, 4.2 kb, and 5.8 kb. Each plasmid contains color-coded gene segments such as AmpR, CMV, Puro, IRES, and others.

Lenti-LTR Packaging Mix (Catalog# LTR-PACK-450/900): The packaging mix for the lentiviral vectors with a wildtype 5’ LTR

Diagram showing two circular DNA plasmids labeled as LTR-PACK-1 and LTR-PACK-2. LTR-PACK-1 is 10.8 kb in size and includes segments labeled 2A, Pgc, AmpR, CAG, pUC, and Pgd. LTR-PACK-2 is 5.8 kb with segments labeled 2A, Pgc, AmpR, PUC, and CMA.

Two helper vectors are included in the optimized packaging mix. One vector provides HIV-1 gag, pol, rev, and tat gene products while the other vector encodes vesicular stomatitis virus G (VSV-G) served as envelope protein